|
KMID : 1234420080360030215
|
|
Korean Journal of Microbiololgy and Biotechnology
2008 Volume.36 No. 3 p.215 ~ p.220
|
|
|
Characteristics of Rahnella aquatilis Strain AY2000 for an Anti-Yeast Substance Production
|
|
Kang Min-Jung
Lee Bok-Kyu
Kim Kwang-Hyeon
|
|
|
Abstract
|
|
|
|
Rahnella aquatilis AY2000 has an unique characteristic which produces an anti-yeast substance (AYS). The AYS of the strain AY2000 was always secreted on agar plate, however, its activity in liquid culture was labile upon storage of the medium. In this paper, cultural conditions of the strain AY2000 for the AYS production were investigated in liquid culture, and minimal inhibitory concentration (MIC) against Saccharomyces cerevisiae was determined for the AYS activity. MIC of the AYS cultured in PYG broth at 25oC for 24 hr was 23.5 ?g/mL, however, that in MYCS (pH 5.5) broth at the same condition was 15.5 ?g/mL. The activity of the AYS had increased rather in MYCS broth excluded NH4-citrate than in the same broth contained NH4-citrate, and MIC of the AYS produced in MYCS broth without NH4-citrate was 15.5 ?g/mL. When the strain AY2000 was maintained in MYCS broth without NH4-citrate but added 100 ?M FeCl3, the activity of the AYS had increased and its MIC was 7.8 ?g/mL. MIC of the AYS was 7.8 ?g/mL after the strain AY2000 was cultured in MYCS broth containing 100 ?M FeCl3 without NH4-citrate, however, its MIC was 31.3 ?g/ mL after 48-60 hr culture in the same broth.
|
|
|
KEYWORD
|
|
|
Anti-yeast substanc, Saccharomyces cerevisiae, Rahnella aquatilis, growth inhibiton, MIC, Liquid culture
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|